![]() In addition, most AAs remained significantly elevated at 6 h post-meal compared with fasted samples across all treatments. ![]() Dogs fed MET had greater plasma and whole blood methionine concentrations from 30 to 360 min after a meal ( P 0.05). Data were analyzed as repeated measures using the PROC GLIMMIX function in SAS (Version 9.4). On day 7, cephalic catheters were placed and one fasted sample (0 min) and a series of nine post-meal blood samples were collected at 15, 30, 60, 90, 120, 180, 240, 300, and 360 min. Eight healthy Beagle dogs were fed one of the three treatments or the control grain-free diet ( CON) for 7 d in a 4 × 4 Latin square design. This study aimed to investigate the effects of supplementing a complete grain-free dry dog food with either methionine ( MET), taurine ( TAU), or methyl donors (choline) and methyl receivers (creatine and carnitine CCC) on postprandial AA concentrations. Although recent work has investigated plasma and whole blood taurine concentrations when feeding grain-free diets, supplementation of a grain-free diet with various nutrients involved in the biosynthesis of taurine has not been evaluated. Pulse ingredients are lower in methionine and cysteine, amino acid ( AA) precursors to taurine synthesis in dogs. Grain-based ingredients are replaced in part by pulse ingredients in grain-free pet foods.
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